Journal: Cell chemical biology

Article Title: 14-helical β-Peptides Elicit Toxicity against C. albicans by Forming Pores in the Cell Membrane and Subsequently Disrupting Intracellular Organelles

doi: 10.1016/j.chembiol.2018.11.002

Figure Lengend Snippet: Key Resource Table

Article Snippet: H 2 O (N-hydroxybenzotrizole monohydrate) Advanced ChemTech Cat # RC8201 Calcein Sigma-Aldrich Cat # C0875 Triethylphosphine oxide Sigma-Aldrich Cat # 562394 (2-(2-(Fmoc-amino)-ethoxy)ethoxy)acetic acid Sigma-Aldrich Cat # 95003 Ergosterol Acros Cat # 117810050 4-chloro-7-nitrobenzofurazan Sigma-Aldrich Cat # 25455 Cyanine5 NHS ester Lumiprobe Cat # 53020 Magainin 2 American Peptide Company Cat # 72-2-26 Melittin American Peptide Company Cat # 86-4-10 TentaGel S RAM Fmoc Advanced ChemTech Cat # SJ5030 Sephadex G-50 Sigma-Aldrich Cat # {"type":"entrez-nucleotide","attrs":{"text":"G50150","term_id":"5221327","term_text":"G50150"}} G50150 Concanavalin A (ConA) Sigma-Aldrich Cat # C2010 1,2-dipalmitoyl- sn -glycero-3-phosphocholine Sigma-Aldrich Cat # P0763 L-α-Phosphatidylglycerol (Egg, Chicken) Avanti Cat # 841138 L-α-phosphatidylethanolamine (Egg, Chicken) Avanti Cat # 840021 L-α-phosphatidylcholine (Egg, Chicken) Avanti Cat # 840051 SYTO 13 Life Technologies Cat # S7575 MDY-64 Life Technologies Cat # Y7536 FM 4–64 Life Technologies Cat # {"type":"entrez-protein","attrs":{"text":"T13320","term_id":"7515243","term_text":"pir||T13320"}} T13320 MitoTracker Green Life Technologies Cat # M7514 Experimental Models: Organisms/Strains C. albicans : SC5314 ATCC MYA-2876 Software and Algorithms pwl2s; piecewise fitting function consisting of two linear segments OriginPro 2018b https://www.originlab.com/doc/Tutorials/Fitting-Piecewise-Linear Zeiss ZEN Zeiss https://www.zeiss.com/ Other Glass bottom cell culture plates Cellvis Cat # D35–20-1.5-N CELLVIEW cell culture dishes Greiner Bio-One Cat # 627861 Open in a separate window Key Resource Table 14-Helical β-peptides can permeabilize synthetic lipid vesicles. β-peptide binding to synthetic vesicles is consistent with a pore formation model. Antifungal β-peptides permeabilize the plasma membrane of Candida albicans . β-Peptides disrupt the nuclear membrane and vacuole to kill fungal cells.

Techniques: Recombinant, Software, Cell Culture